- Gargamel from The Smurfs
- Mok Swagger from Rock and Rule
- Ruber from Quest for Camelot
- Rasputin from Anastasia
- Eris from Sinbad: Legends of the Seven Seas
- Mr. Swackhammer from Space Jam
- Carface Curruthers from All Dogs Go to Heaven
- Jenner from The Secret of NIMH
- Martin Brisby from The Secret of NIMH 2: Timmy to the Rescue
- Yosemite Sam from The Looney Tunes
- Warren T. Rat from An American Tail
- Cat R. Waul from An American Tail: Fievel Goes West
- Professor Screweyes from We're Back: A Dinosaur's Story!
- Cardinal Richelieu from Dogtanian and the Three Muskhounds
- Count Rochefort from Dogtanian and the Three Muskhounds
- Injurin' Joe from Tom Sawyer
- Steele from Balto
- Bluto from Popeye
- General Woundwort from Watership Down
- Claudandus from Felidae
- Prince Froglip from The Princess and the Goblin
- Ali Cat from Garfield on the Town
- Harry the Heartless from Mighty Mouse in the Great Space Chase
- Ogthar from Dinotopia: Quest for the Sunstone
- Blackwolf from Wizards
- Zygon from Starchaser: The Legend of Orin
- Dr. Claw from Inspector Gadget
- Buzz Buzzard from The New Woody Woodpacker
- Sharptooth from The Land Before Time
- The Snow Queen from The Snow Queen
- Fleshlumpeater from The BFG
- The Prince from Romeo and Juliet: Sealed with a Kiss
- Count Beajeaux from The Return of Dogtanian
- Julius Caesar from Asterix Conquers America
- The Grand Duke of Owls from Rock-a-Doodle
- Simone Lenoir from Scooby-Doo on Zombie Island
- The Mouseking from The Nutcracker Prince
- Napoleon from Animal Farm
- The Joker from Batman: Mask of the Phantasm
- The Red Bull from The Last Unicorn
- Rothbart from The Swan Princess
- Scar Snout from The Rugrats Movie
- The Spirit of the Book from The Care Bears Movie
- Transfer from Around the World in Willy Fog
- Mr. Burns from The Simpsons
- Laird from The Princess and the Pea
- The Swarm Lord from The Magic Voyage
- Nicholas Cherrywood from The Care Bears Movie
- General Wouldward from Watership Down
- Meowrice from Gay Purr-ee
- The Collage of Crooks from Cool McCool
- Muriel and Floyd from The Secret of NIMH 2: Timmy to the Rescue
- Pit Pat and Pot from The World in David the Gnomes
- Darla Dimple from Cats Don't Dance
- Max from Cats Don't Dance
- Mr. Grasping Toplofty and O'Bloat from An American Tail: The Treasure of Manhatten Island
- Madame Mousey from An American Tail: The Mystery of the Night Monster
- Mr. Slate from The Flintstones
- Dr. Greed from The Fearless Four
- Plankton from The SpongeBob SquarePants Movie
- The Barn Owl from Once Upon a Forest
- Aunt Pristine Figg from Tom and Jerry: the Movie
- Mr. Lickboot from Tom and Jerry: the Movie
- Darkheart from Care Bears Movie 2: A New Generation
- The Wizard of Wonderland from The Care Bears: Adventures in Wonderland
- Widimer from Dogtanian and the Three Muskhounds
- The Pirate Ghosts from Garfield's Halloween Adventure
- El Supremo from Freddie as F.R.O.7
- Tzekel-Khan from The Road to El Dorado
- The Widow from The Magic Riddle
- Nekron from Fire & Ice
- The Emperor of the Night from Pinocchio and the Emperor of the Night
- Grundel Toad from Thumbelina
- Zigzag the Guard Vizier from The Thief and the Cobbler
- The Weasel from Garfield and Friends
- Dangerous Dan from Heckle and Jackle
- Team Rocket from Pokemon
- Devimon from Digimon
- Lord Balthazar from The Smurfs
- Coco LaBouche from Rugrats in Paris the Movie
- Megatron from Transformers the Movie
- Lord Maliss from Happily Ever After
- Joe from Help I'm a Fish
- Baron Slias Greenback from Danger Mouse
- Dick Dastardly from Dastardly And Muttley In Their Flying Machines
- Thrax from Osmosis Jones
- Mojo Jojo from The Powerpuff Girls Movie
- Messina from Freddie as F.R.O.7
- Hexxus from FernGully: The Last Rainforest
- Kent Mansley from The Iron Giant
- Puppetino from Pinocchio and the Emperor of the Night
- Stormella from Rudolph the Red-Nosed Reindeer the Movie
- The Drej Queen from Titan A.E.
- Queen Gnorga from A Troll in Central Park
- Pinky and the Brain from Pinky and the Brain
- Holli Would from Cool World
- The Dragon from The Pagemaster
- Tyler from Heavy Metal 2000
- King Haggard from The Last Unicorn
- The Duke of Zill from Felix The Cat the Movie
- Pharaoh Rameses from The Prince of Egypt
- Mr. Beetle from Thumbelina
- Drake from The Pebble and the Penguin
- Larson Petty from ALF
- Red from All Dogs Go to Heaven 2
- Belladonna from An All Dogs Christmas Carol
- Caractacus P. Doom from Avenger Penguins
- Sir Oswald from Sooty's Amazing Adventures
- King Bombo from Gulliver's Travels
- Monsieur Grochoux from 1001 Gags of Stiff and Hercules
- Hydia from My Little Pony the Movie
- The Wicked Witch of the East from Lion of Oz
- The Monstars from Space Jam
- Dennis from The SpongeBob SquarePants Movie
- Russ Cargill from The Simpsons Movie
- The Demon from Rock and Rule
- The Great Animal from The Swan Princess
- The Nightmare King from Little Nemo: Adventures in Slumberland
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Non Disney Villains Tribute (Manuel Lobato Humanes Version)
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